Ctd110.6 antibody

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August undefined, 2024

WebJul 23, 2024 · O-GlcNAc Antibody (CTD110.6) is a mouse monoclonal IgM κ O-GlcNAc antibody, cited in 62 publications, provided at 200 µg/ml; raised against O-GlcNAc …

O-GlcNAc (CTD110.6) Mouse mAb Cell Signaling …

WebMonoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 … WebSimilar results were obtained for wild-type HEK293T cells (not shown). (C) Expression and O-GlcNAcylation of Notch1, Notch1Δ2xO-GlcNAc and Notch1Δ4xO-GlcNAc were analyzed by immunoprecipitation (IP) using NOTCH1 (8G10) antibody, followed by immunoblotting with CTD110.6 O-GlcNAc or NOTCH1 antibodies. list of law college in bangladesh

Identification of O‐GlcNAcylated Proteins in Human Platelets

WebJun 15, 2001 · The antibody reacts with a large number of proteins from cytoplasmic and nuclear extracts and readily detects in vivo changes in O-GlcNAc modification. These … WebMar 5, 2024 · Compared to immunostaining of O-GlcNAcylated proteins by CTD110.6 antibody, OGT immunostaining appears to be more cytosolic with less intense immunoreactivity in hippocampal CA1 (ratios of immunoreactivity intensity between CA1 to adjacent region are ~ 0.73 for OGT and ~ 2.5 for CTD110.6, respectively) or dentate … Webwww.ncbi.nlm.nih.gov list of law clerks supreme court

CD46 Polyclonal Antibody (PA5-35311) - Thermo Fisher Scientific

Western blot data using two distinct anti- O-GlcNAc monoclonal

WebO-GlcNAc antibody (mAb) (Clone CTD110.6) for use in Immunoprecipitation (IP) ... This antibody recognizes single O-GlcNAc residues in b-O-glycosidic linkage to serine and … WebJan 1, 2015 · The specificity of the CTD110.6 antibody immunoreactivity was confirmed by a hapten inhibition test using 0.1 m GlcNAc. B, the levels of O-GlcNAc modification on N1EGF1–36-MycHis quantitated by densitometric analyses of immunoblots represented in A (n = 3; results are shown as mean ± S.D.; *, p < 0.01; **, p < 0.05; unpaired Student's t … imdb 12 o\\u0027clock highWebMay 18, 2024 · 3. Incubate the blots with CTD110.6 (diluted to 1 μg/ml in antibody dilution buffer), in duplicate, with and without 100 mM GlcNAc, overnight at 4°C. An antibody that has been antigen purified using a GlcNAc-agarose column yields higher-quality blots. Commercially available CTD110.6 is purified by ammonium sulfate precipitation. imdb 11th hour

"WebCTD110.6, 18B10 and 9D1 are anti-O-GlcNAc monoclonal antibodies. B. HEK 293T cells were treated with DMSO, 50 μM TG or 50 μM 5SG for 24 hours, 200 nM rapamycin … " - Ctd110.6 antibody

Ctd110.6 antibody

WebThe β-O-linked N-acetylglucosamine (O-GlcNAc) antibody recognizes glycosylation, a post-translational modification, which occurs on serine and threonine residues of proteins in. Ximbio. Find reagents. ... Anti-O … http://bb-china.net/search.html?brand=42&pcategory=0&classd=0&use=0&kw=&promotions=2&inventory=2&category=17&page=21

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WebMonoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 cells) and cells from BALB/c mice immunized with a synthetic peptide YSPTS(O-GlcNAc)PSK conjugated to KLH.This antibody recognizes O-GlcNAc in β-O-glycosidic … WebFor O-glcNAcylation detection, CTD110.6 antibody was covalently conjugated to (4) Defective STIM-mediated store operated Ca2+ entry in hepatocytes leads to metabolic dysfunction in obesity eLife December 15, 2024 Ana Paula Arruda, Benedicte Mengel Pers, Günes Parlakgul, Ekin Güney, Ted Goh, ...

WebIn 2008, the Okajima group originally discovered the O-GlcNAc modification on Drosophila Notch EGF20 by detecting O-glycans on the ECD of Drosophila Notch using an antibody that recognizes O-GlcNAc (CTD110.6) . Later, O-GlcNAc modifications of the EGF repeats in Drosophila Delta, Serrate, and Dumpy were subsequently discovered [86,87]. WebCTD110.6 : Host: Mouse : Isotype: IgM : Immunogen: Synthetic peptide containing serine-O-GlcNAc. Species reactivity: Species independent : Specificity: Recognizes Ser-O-GlcNAc and Thr-O-GlcNAc in eukaryotes. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. Crossreactivity:

WebNov 22, 2024 · Results indicate Cdcp1 (CD318) as a ligand of T-cell differentiation antigen CD6 (CD6) and a potential target for the diagnosis and treatment of … WebThus, with careful controls, antibodies CTD110.6 (IgM), #3 (IgG), and #10 (IgG) can be used to detect membrane and secreted proteins modified by O-GlcNAc on EGF repeats. Secreted and cell surface glycoproteins carry a wide spec-trum of N-and O-glycans that are synthesized during transit

WebDec 9, 2016 · The RL2 epitope was detected on Sp1 during 2DG treatment, and the level was transiently increased at 24 h. In contrast, the CTD110.6 epitope became detectable on Sp1 over 72 h after 2DG treatment, and then the other proteins containing CTD110.6 epitopes also appeared in the cell lysates and the anti-Sp1 antibody precipitates.

WebThe β-O-linked N-acetylglucosamine (O-GlcNAc) antibody recognizes glycosylation, a post-translational modification, which occurs on serine and threonine residues of proteins in. Ximbio. Find reagents. ... Anti-O-GlcNAc (CTD110.6) antibody Invented at ... imdb 12 hours to liveWebApr 19, 2011 · Results. When T24 human bladder cancer cells were subjected to glucose deprivation, the expression of proteins detected by the O-GlcNAc specific antibody … list of law enforcement agencies in michiganWebCTD110.6 : Host: Mouse : Isotype: IgM : Immunogen: Synthetic peptide containing serine-O-GlcNAc. Species reactivity: Species independent : Specificity: Recognizes Ser-O-GlcNAc … list of law firm in cambodiaWebO-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation. PLoS One. 2011 04 19;6(4):e18959. Zachara, Natasha E; Molina, Henrik; Wong, Ker Yi; Pandey, Akhilesh; Hart, Gerald W. The dynamic stress-induced "O-GlcNAc-ome" highlights functions for O-GlcNAc in regulating DNA … imdb 12 hour shiftWebO-GlcNAc (CTD110.6) is a mouse monoclonal antibody raised against a peptide containing serine-O-linked N-acetylglucosamine. PRODUCT Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of list of law enforcement agencies in nigeriaWebFormulation. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA. Preparation. The antibody was purified by chromatography and conjugated with … imdb 13 hours the secret soldiers of benghaziWebApr 24, 2024 · Among these antibodies, CTD110.6 and RL2 are the most widely used. However, due to a certain degree of amino acid sequence dependency, these antibodies cannot detect all proteins with O-GlcNAcylation. Additionally, a few antibodies for site-specific protein O-GlcNAcylation have been reported, ... imdb 127 hours