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WebEGF contribution (60 %) (EUR) 5 019 218 ASSESSMENT OF THE APPLICATION Procedure 4. On 22 December 2024, the Netherlands submitted application EGF/2024/004 NL/KLM, i.e. within 12 weeks of the date on which the intervention criteria set out in Article 4 of the EGF Regulation were met. The Commission WebImmunofluorescence analysis of signal transducer and activator of transcription 3 (STAT3) was performed using A549 cells treated with 100 ng/mL human EGF recombinant protein ( Product # PHG0311) for 30 minutes. The cells were labeled with phospho-STAT3 (Ser727) recombinant rabbit monoclonal antibody ( Product # MA5-33199) (Panel a & c; Green). Web13 mei 2015 · Cells were seeded at a density of 4000 cells/well in duplicate or triplicate in 96 well plates and incubated overnight at 37°C in the full media with or without EGF (20 ng/ml). After 24 hours, the media was replaced with media (with or without EGF) containing serial dilutions of gefitinib. can the human intestines go around the earth